Brenda K Minesinger Email Address

Senior Principal Scientist

Intellia Therapeutics is a leading gene-editing company focused on the development of curative medicines using CRISPR/Cas9 technology

Highly competent, productive, and innovative biologist with a versatile skill set encompassing molecular biology, cell biology, microbiology and biochemistry. Committed to timely data delivery. Demonstrated leader, manager and team player who fosters strong working relationships within interdisciplinary research groups and across management levels. Respected public speaker and scientific writer with a talent for effectively communicating key concepts of complex biological problems to both laypeople and experts in the field. Technical Specialties: * DRUG DISCOVERY: Design and troubleshooting of small-molecule inhibitor screens; proof-of-principal studies; target validation; hands-on assay development * MOLECULAR BIOLOGY: Phage amplification, precipitation/concentration, viral titer quantification, and phage purification by CsCl gradient ultracentrifugation. Plasmid and genomic DNA manipulation, Seamless/Gibson cloning, gene cloning from bacteria, fungi and mammalian cells, site-directed mutagenesis, nucleic acid detection and amplification, cDNA amplification, qRT-PCR, DNA sequence analysis, RNA isolation, Northern blotting * TISSUE CULTURE (HEK 293T, U2OS, PC12): Cell line maintenance, transfection optimization, plasmid transfection, assessment of protein expression, live and fixed cell fluorescent imaging, co-immunoprecipitation, RNAi knock downs and stable cell line generation * GENETICS: Knock-ins and knock-outs of genes in fungal genomes (S. pombe and S. cerevisiae); S. pombe mating for genomic manipulation; Familiar with library preparation and sequencing technologies * BIOCHEMISTRY: Recombinant protein expression in E. coli, protein isolation and separation by ion-exchange chromatography and gel filtration. Protein interaction studies, including co-immunoprecipitation, peptide array and Western Blotting